The present study was carried out to evaluate the antidiabetic effect of Selaginella bryopteris extract and to study the activities of Blood Glucose, Total cholesterol, Triglyceride, Amylase, Creatinine, Urea, Uric acid in different group of normal, alloxan induced diabetic, insulin alloxan induced diabetic and Selaginella bryopteris alloxan induced diabetic. Alloxan (120mg/dl) was administered intra-peritoneal to swiss albino mice and after establishment of diabetes, aqueous extract of Selaginella bryopteris (150mg/kg b.w) was orally administered for 26 day. It was noticed that Selaginella bryopteris reduced the level of glucose, total cholesterol, triglyceride, amylase, creatinine, urea and uric acid in mice.