Lignocellulose has a complex structure composed mainly of lignin, hemicellulose and cellulose. Several enzymes are needed for the degradation of lignocellulose into simple sugars to produce
bioethanol and it have economically valuable (Lynd, et al., 1991). Novel enzymes can be discovered from environmental isolates that are known to produce a diversity of enzymes. Soil samples were collected from the rizosphere zone of litchi garden of BRA, Bihar University Muzaffarpur, Bihar, India and 20 different actinomycetes stains were isolated and it was observed that extracellular enzymes can degrade different lignocellulolosic substrates such as CMC, Xylan, Lignin and Mannan. It was also observed that out of total isolates most of the isolated strains belong to the genus i.e., 70% Streptomyces, followed by 25% Actinobacterium and 5% Nocardia. Analysis of the 16S rRNA gene sequences and phylogenetic tree construction of the strains showed that strain SGIITD05 and SGITD02 had the highest sequence similarity i.e., 99% to Streptomyces spLD48 and Streptomyces spA515Ydz-FQ respectively.