In the present study, a correlation has been made by using polynomial regression between the protein productions against the treatment of different concentration of Gibberllin in perisperm of Euryale ferox Salisb. After 0.001% Gibberellin treatment on perisperm for 1min and 5min leads to higher concentration i.e., 50.08μg/mg protein was observed in content of the kernel on mature fruits as compared to 1/3rd mature fruits after of treatment with respect to lower protein production at 0.0001% and 0.01% Gibberellin treatment. The highest concentration was observed after 236 day after showing (DAS).